In Smurf Village, the Smurfs live peacefully among themselves. Their leader is the wise, elderly, and a bit overprotective, Papa Smurf. Some of the Smurfs include the klutzy and slow-witted Clumsy Smurf, the intelligent and eccentric Brainy Smurf, the strong and gutsy Hefty Smurf and the beautiful Smurfette, who feels out of place in Smurf Village as the only girl. Smurfette was created by the wicked wizard Gargamel from a lump of clay, but was redeemed by Papa Smurf and became part of the village. Gargamel makes it his mission to capture the Smurfs, steal all of their essences, and become the most powerful wizard in the world.
After Smurfette accidentally breaks an invention by Brainy, made up by a fun day snowboarding with Brainy, Hefty, and Clumsy, Smurfette sees a blue creature hidden by a leaf and follows it straight into the Forbidden Forest, which Papa Smurf forbids the Smurfs from entering, believing the creature to be a Smurf. She loses sight of the creature but she discovers a hat that was dropped by the possible Smurf. Smurfette ends up being captured by Gargamel and at his castle, she ends up inadvertently revealing the hat, enabling Gargamel to mix up a brew that causes him to locate the village on a map. Soon enough Hefty, Brainy, and Clumsy, who followed Smurfette, help her escape and return to Smurf Village, where they end up being punished by Papa Smurf for disobeying his orders and he confines them to their houses while dismissing their claims of a lost village filled with Smurfs.
However, Smurfette, still wanting to find her place, sneaks out into the night, with Brainy, Clumsy, and Hefty volunteering to come with her, simply because they’re Team Smurf. Smurfette agrees to let them join her and together they enter the Forbidden Forest. Gargamel soon discovers them trying to find the lost village and heads out with his cat Azrael and giant bird Monty to stop them.
The four best friends follow the map and end up in various adventures, such as being attacked by monster flowers and getting caught in a stampede of luminescent rabbits, one of whom they befriend and name Bucky, who helps them on their quest. After setting sail on their handmade raft along the river, they encounter Gargamel, Azrael, and Monty. Following a brief chase, Gargamel is thrown out of his own raft. Hefty and Smurfette convince the others to help save him. They do so, but instead of thanking them, he pushes them out of their raft, leaving them to plunge down a waterfall.